BIO 357 - Biotoxicity of Hookah Smoke

Students will perform experiments that determine how altering the pattern of smoking (puff topography) and shisha flavor change the resulting smoke’s effect on cultured lung cells by quantifying viability metrics (lysosomal function and mitochondrial function) and inflammation (cytokine expression) after smoke exposure. To be better able to interpret our results discussion topics include epithelial tissue composition, the fundamental barrier between inhaled air and the body, smoke components, and the biochemistry behind the viability assays we use. The course includes mammalian cell culture maintenance, data collection and statistical analysis, oral presentations, preparing and presenting a scientific poster, and writing a research article.

 

Prerequisites & Notes
Successful completion of CHE 115, BIO 111/113/115, and at least one BIO 200+ course are required.

Instructor permission is required to register for the course.